Research Technician II-III, Single Cell Sequencing
Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch’s pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation’s first cancer prevention research program, as well as the clinical coordinating center of the Women’s Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.
At Fred Hutch, we believe that the innovation, collaboration, and rigor that result from diversity and inclusion are critical to our mission of eliminating cancer and related diseases. We seek employees who bring different and innovative ways of seeing the world and solving problems. Fred Hutch is in pursuit of becoming an antiracist organization. We are committed to ensuring that all candidates hired share our commitment to diversity, antiracism, and inclusion.
The Fred Hutch Innovation Laboratory (FHIL) at seeks an enthusiastic and highly motivated technician to join a team of scientists working in a multi-disciplinary environment on the forefront of fundamental and immunotherapy research. FHIL is a new collaborative initiative of the Immunotherapy and Translational Data Science Integrated Research Centers (IIRC and TDS_IRC) with a mission to advance the introduction and development of new immuno-oncology research technologies. FHIL will spur in-house development and early adoption of novel techniques, including advanced single cell RNA spatial sequencing, T- and B-cell receptor sequencing, and other DNA/RNA-based technologies.
The successful candidate will test new technologies, compare and develop protocols and use these technologies to support ongoing research in immune-oncology. This person will be responsible for comparing and improving protocols, generating data to compare technologies, benchmarking technologies in collaboration with computational biology collaborators. The technician will perform bench-level experiments, operate analytical instruments, and compile and verify the accuracy of experimental data. The technician will also maintain detailed laboratory records, analyze data, and report on experimental results.
This position requires a high level of organization, a keen eye for detail, and accurate and thorough record keeping. We seek a candidate who is consistent in executing lab protocols and can swiftly adapt and troubleshoot new ones. This position will be extended to a candidate who is creative, passionate, persistent, patient, and critical, with a strong sense of independence, initiative, and drive to propel work forward. We seek a committed candidate who is an effective communicator of science; who is confident and proactive, with excellent interpersonal skills, discipline, readiness to learn and contribute, and eagerness to take on new challenges.
Responsibilities
- Compare and refine protocols for single-cell RNA sequencing and other DNA/RNA based technologies.
- Generate data to compare new technologies; many of these technologies will focus on single cell techniques but are not limited to this area.
- Maintain laboratory records of all testing and protocol development and report on the performed work as necessary.
- Perform bench experiments, generate sequencing libraries, and compile other components of the downstream analysis of large experiments for immunotherapy researchers.
- Maintain laboratory stocks, cultures, cell lines, reagents, and other supplies.
- Manage a research database and maintain detailed electronic records.
- Conduct literature reviews to assist in experimental design, including the identification of alternate methods.
- Assist in the preparation of scientific manuscripts by contributing ideas, proofreading, and copy editing.
Qualifications
Required:
- This is an intermediate to senior level position requiring at least 2 years' previous experience working in a laboratory and BA/BS in Biology or a related field.
- Research Tech II level: a bachelor's degree in a biological field and 2 or more years of relevant professional post-bachelor's laboratory research experience
- Research Tech III level: Extensive laboratory experience. Generally, the incumbent will possess a Master's degree in a related scientific field.
- Independent research experience outside of laboratory course work is necessary.
- 1+ years' professional post-graduate experience in next generation sequencing (NGS)
- Experience in genomics
- A strong background in molecular and cellular biology, including cloning, transfection/infection of cells, and PCR is essential
Preferred:
- Experience in single cell RNA-seq, tissue culture, QPCR, ddPCR, FACs, fresh frozen and FFPE tissue sectioning is highly desirable.
Our Commitment to Diversity
We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our HR Operations at HRops@fredhutch.org or by calling 206-667-4700.