Post-Doctoral Research Fellow - Pancera Lab
Cures Start Here. At Fred Hutchinson Cancer Research Center, home to three Nobel laureates, interdisciplinary teams of world-renowned scientists seek new and innovative ways to prevent, diagnose and treat cancer, HIV/AIDS and other life-threatening diseases. Fred Hutch’s pioneering work in bone marrow transplantation led to the development of immunotherapy, which harnesses the power of the immune system to treat cancer. An independent, nonprofit research institute based in Seattle, Fred Hutch houses the nation’s first cancer prevention research program, as well as the clinical coordinating center of the Women’s Health Initiative and the international headquarters of the HIV Vaccine Trials Network. Careers Start Here.
At Fred Hutch, we believe that the innovation, collaboration, and rigor that result from diversity and inclusion are critical to our mission of eliminating cancer and related diseases. We seek employees who bring different and innovative ways of seeing the world and solving problems. Fred Hutch is in pursuit of becoming an antiracist organization. We are committed to ensuring that all candidates hired share our commitment to diversity, antiracism, and inclusion.
The Pancera/Stamatatos lab, within the Vaccine and Infectious Disease Division, works toward the development of safe and effective vaccines against HIV-1, malaria and other pathogens. Our major emphasis is the design of protein immunogens and understanding the immune response following vaccination or natural infection. The subgroup led by Dr. Pancera focuses on structural characterization of these immunogens and their immune responses. The laboratory utilizes a wide range of in vitro and in vivo systems and cutting-edge technologies and techniques. Experimental approaches include high-throughput crystallography, cryoEM, specialized immunological methodologies and assays, molecular and cellular biology techniques, bioinformatics, and computational approaches.
Our lab is seeking an outstanding Postdoctoral Fellow to work on a project involving the characterization of the humoral immune response following vaccination against HIV, malaria or other pathogens using X-ray crystallography and cryoEM and the designs of novel immunogens.
Responsibilities
- Perform recombinant protein expression (coli, insect cells, mammalian cells) and purification including affinity purification, ion exchange and FPLC
- Determine X-ray crystal structures of the proteins/antibodies complexes
- Use Electron Microscopy for screening purposes (negative stain) and single particle cryoEM to determine the molecular structures of antigen-antibody complexes
- Use of biophysical methods such as ITC and Bio Layer interferometry/SPR or ELISA
- Initiate and carry out scientific projects under the supervision of Dr. Pancera
- Develop and demonstrate a thorough understanding of the scientific literature relating to the research topics
- Conduct and publish research in HIV structural biology
Qualifications
- PhD in Structural Biology, Protein Chemistry, Biochemistry or a related field is required with significant expertise in X-ray protein crystallography
- Candidates with backgrounds in areas such as protein biochemistry, and vaccine development are strongly encouraged to apply
- On-hands experience with diverse aspects of Molecular Biology is required
- Experience in X-ray crystallography including up-to-date knowledge of relevant programs including CCP4, and Phenix packages, protein purification, protein characterization, and a strong publication history are strongly desired
- Knowledge of cryoEM is not required but desirable. Additional expertise with tissue culture and immunological assays is a plus
- The candidate must be a ‘risk taker’, show initiative, willingness to take up new skills and responsibilities, and be a team player
- Excellent attention to details and the ability to work independently are essential to success in this position, as are good communication and organizational skills
- The candidate must be self-motivated, able to multitask and to work within a very collaborative environment
- Salary will be commensurate with (appropriate) experience
- A cover letter and research statement is required
A statement describing your commitment and contributions toward greater diversity, equity, inclusion, and anti-racism in your career or that will be made through work at Fred Hutch is requested of all finalists.
Our Commitment to Diversity
We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to our Employee Services Center at hrops@fredhutch.org or by calling 206-667-4700.